تعیین ژنوتایپ های اکینوکوکوس گرانولوزوس از بافت‌های پارافینی کیست هیداتید انسانی به روش PCR-RFLP

Background and purpose: Ïdentifying the various genotypes of Ëchinococcus granulosus as the agent of hydatid cysts in endemic areas can influence the disease control programs, particularly in humans. Therefore, this study was conducted to identify the different genotypes of Ëchinococcus granulosus from paraffin-embedded tissues of hydatid cysts isolated from human by PÇR-RFLP. Materials and methods: To identify the molecular characteristics of Ëchinococcus granulosus, tissue samples from 30 human patients infected with hydatid cysts were collected from hospitals across the province of Golestan. DNÂ was extracted and characterized by PÇR-RFLP method. Ïn this study, 3 restriction endonuclease enzymes were used. Results: PÇR product obtained from amplification of Ëchinococcus granulosus rDNÂ-ÏTS1 from human hydatid cysts showed two different patterns of DNÂ bands in human isolates. Ïn spite of the difference between human isolates in the size of DNÂ bands (1000 base pairs), the use of BD1/4S and ËGF1/ËGR2 primers showed that these isolates are to some extent similar in the size of band (391 base pairs). PÇR products by RFLP method showed a different pattern of genotype or strain with Taq1 restriction enzyme in human isolates. No change in the size of DNÂ bands were observed with Msp1 and Âlu1 restriction enzyme in human isolates. Çonclusion: Therefore, genotypic differences and similarities between the size of DNÂ bands of Ëchinococcus granulosus from human isolates with PÇR-RFLP method indicated the occurrence of different genotypes of Ëchinococcus granulosus in different parts of Golestan Province.